CD30L expression in a cultured TEC clone derived from postnatal human thymus. (A) Immunostaining for cytokeratin of cultured cells from a thymic clone; cells were fixed in acetone and stained by using the avidin-biotin-peroxidase method and the AEC substrate (red color, original magnification ×100); inset: high-power magnification of three cells showing intense cytokeratin immunoreactivity (original magnification ×250). (B) Absence of reactivity by the same cells stained with an isotype-matched control MoAb (original magnification ×100). (C) Immunostaining for CD30L of cultured epithelial cells from the same postnatal thymus clone; cells were fixed in 4% paraformaldehyde and staining was performed by using the avidin-biotin-peroxidase method and the AEC substrate (red color, original magnification ×100). (D) Detection of CD30L expression on cultured cells from the same postnatal thymus clone by flow cytometry. Cells (1 × 106/mL) were resuspended in PBS containing 0.5% BSA and 0.02% sodium azide and incubated with anti-CD30L (black area) or isotype-matched control (white area) MoAb, followed by FITC-conjugated anti-mouse IgG2b goat Ab. Absence of CD30L expression in cultured kidney glomerular epithelial cells (E) and in cultured T lymphocytes obtained from the same postnatal thymus (F).
