American Society of Hematology

Fig. 3.

$Fig. 3. RT-PCR analysis of amphotropic receptor (ampho R) mRNA levels in fresh and frozen human cord blood cells. The levels of mRNA encoding ampho R mRNA and β2-microglobulin mRNA in unfractionated (UF) Lin− CD34+ CD38− and Lin− CD34+ CD38+ cells were analyzed as in Fig 2. The level of ampho R mRNA in the HSC-enriched Lin− CD34+ CD38− fraction from frozen cord blood is significantly increased (P < .005) over the level of ampho R mRNA in the HSC-enriched Lin− CD34+ CD38−fraction from fresh cord blood (see Table 2).$

RT-PCR analysis of amphotropic receptor (ampho R) mRNA levels in fresh and frozen human cord blood cells. The levels of mRNA encoding ampho R mRNA and β2-microglobulin mRNA in unfractionated (UF) Lin⁻ CD34⁺ CD38⁻ and Lin⁻ CD34⁺ CD38⁺ cells were analyzed as in Fig 2. The level of ampho R mRNA in the HSC-enriched Lin⁻ CD34⁺ CD38⁻ fraction from frozen cord blood is significantly increased (P < .005) over the level of ampho R mRNA in the HSC-enriched Lin⁻ CD34⁺ CD38⁻fraction from fresh cord blood (see Table 2).

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