American Society of Hematology

Fig. 5.

$Fig. 5. RT-PCR analysis of amphotropic retrovirus receptor mRNA levels in BM hematopoietic stem cells from untreated mice (top panel), and from mice injected for 5 consecutive days with G-CSF and SCF (bottom panel). BM cells were collected from mice treated with G-CSF and SCF at 14 days after the last cytokine treatment. The cells were fractionated by CCE at flow rates of 25, 30, and 35 mL/min (FR25, FR30, FR35), and Lin− c-kitHI cells were isolated from each fraction by flow cytometry. The levels of amphotropic receptor mRNA were quantified based on the level of β2-microglobulin mRNA (not shown) in the same sample. These values were normalized to the level of amphotropic receptor mRNA in NIH-3T3 cells (right column) and compared with the mRNA level in unfractionated BM (see Table 3).$

RT-PCR analysis of amphotropic retrovirus receptor mRNA levels in BM hematopoietic stem cells from untreated mice (top panel), and from mice injected for 5 consecutive days with G-CSF and SCF (bottom panel). BM cells were collected from mice treated with G-CSF and SCF at 14 days after the last cytokine treatment. The cells were fractionated by CCE at flow rates of 25, 30, and 35 mL/min (FR25, FR30, FR35), and Lin⁻ c-kit^HI cells were isolated from each fraction by flow cytometry. The levels of amphotropic receptor mRNA were quantified based on the level of β2-microglobulin mRNA (not shown) in the same sample. These values were normalized to the level of amphotropic receptor mRNA in NIH-3T3 cells (right column) and compared with the mRNA level in unfractionated BM (see Table 3).

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