Nucleotide sequence of cDNAs encoding CKβ8-1 and CKβ8 and the deduced amino acid sequence. The nucleotide sequence of the message strand is numbered in the 5′ to 3′ direction. The 5′ UTR sequence is indicated by negative numbers. The predicted amino acid sequence is shown below the nucleotide sequence. Underlined are the putative signal peptides. The stop codons are indicated by a star (*). The six cysteine residues are depicted in boxes. These sequences have been deposited in the GenBank data base (Accession No. U58913 andU67128 for CKβ8-1 and CKβ8, respectively). (A) The CKβ8-1 cDNA sequence is shown. Seventeen amino acids (Leu⁴⁷ to Gly⁶³) created by alternative splicing are represented by a large box. In addition, Met⁴⁶ is denoted by a filled circle (•) that was converted to Arg⁴⁶ in CKβ8 due to the alternative splicing. (B) The CKβ8 cDNA sequence is shown. Depicted by a filled circle (•) is Arg⁴⁶, which was derived fro m Met⁴⁶ of CKβ8-1.