Fig. 2.
Fig. 2. Myc mRNA and protein expression in Ba/F3 cells expressing or not TEL/PDGFRβ. (a) Cells were cultured for 24 hours in the presence (+) or in the absence (−) of IL-3. Cell lysates were analyzed by Western blotting using an anti-Myc antibody. (b) Total RNA from Ba/F3 cells transfected with either the control vector or TEL/PDGFRβ and cultured for 18 hours without IL-3 were analyzed by Northern blotting using an Myc cDNA probe. Lower panel is ethidium bromide staining of the 28S ribosomal RNA used as a control of RNA concentration in each lane. (c) Untransfected Ba/F3 cells as well as TEL/PDGFRβ-expressing cells were cultured for 18 hours in the presence (+) or in the absence (−) of IL-3, respectively, with (+) or without (−) 0.3 μmol/L of CGP53716. Cell lysates were analyzed by Western blotting using an anti-Myc antibody.

Myc mRNA and protein expression in Ba/F3 cells expressing or not TEL/PDGFRβ. (a) Cells were cultured for 24 hours in the presence (+) or in the absence (−) of IL-3. Cell lysates were analyzed by Western blotting using an anti-Myc antibody. (b) Total RNA from Ba/F3 cells transfected with either the control vector or TEL/PDGFRβ and cultured for 18 hours without IL-3 were analyzed by Northern blotting using an Myc cDNA probe. Lower panel is ethidium bromide staining of the 28S ribosomal RNA used as a control of RNA concentration in each lane. (c) Untransfected Ba/F3 cells as well as TEL/PDGFRβ-expressing cells were cultured for 18 hours in the presence (+) or in the absence (−) of IL-3, respectively, with (+) or without (−) 0.3 μmol/L of CGP53716. Cell lysates were analyzed by Western blotting using an anti-Myc antibody.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal