Myc expression and cell growth of TEL/PDGFRβ Ba/F3 cells expressing or not MycRX. (a) Protein extracts from Ba/F3 cells either transfected with TEL/PDGFRβ or cotransfected with TEL/PDGFRβ and MycRX were analyzed by Western blotting using an antibody directed against the C-terminal part of Max. (b) TEL/PDGFRβ-transfected Ba/F3 cells expressing or not expressing MycRX were cultured for 24 hours with 1% or 10% FCS, and cell lysates were analyzed by Western blotting using an Myc antibody directed against the N-terminal part of Myc. (c) TEL/PDGFRβ-transfected Ba/F3 cells expressing or not expressing MycRX were seeded at a density of 105 cells/mL in the absence of IL-3 and in the presence of either 1% or 10% FCS. Cell numbers were determined at different times of culture. Data represent the mean ± SD of three independent experiments (*P < .0001 v TEL/PDGFRβ). (▪), TEL/PDGFRβ; (•), TEL/PDGFRβ/MycRX.
