Fig. 4.
Effect of hypoxia, carbon monoxide, cobalt chloride, and Tiron on the levels of uPAR mRNA in HTR-8/SVneo cells. Cells were cultured for 24 hours under the conditions listed over each lane of the figure. Levels of uPAR mRNA were increased 2.8, 1.8, 3.5, and >10-fold, respectively, within cells cultured under hypoxic (1% O2) conditions, hypoxic conditions in the presence of 20% carbon monoxide, and standard (20% O2) conditions in the presence of either cobalt chloride or Tiron, in comparison to cells cultured under standard conditions alone (lane 1). Carbon monoxide reduced the hypoxia-mediated increase in uPAR mRNA by 35%.
