American Society of Hematology

Fig. 4.

$Fig. 4. Recovery of Gr B and TPH expression after the introduction of +-MITF cDNA into mi/mi CMCs. Five micrograms of total RNA from +/+ CMCs, mi/mi CMCs, and mi/miCMCs overexpressing +-MITF were fractionated on 1% agarose gels and subjected to hybridization with the MITF, Gr B, and TPH probes. The +-MITF cDNA introduced into mi/mi CMCs appeared to be transcribed abundantly. Reprobing with the β-actin and MC-CPA probes showed that equal loading had been achieved.$

Recovery of Gr B and TPH expression after the introduction of +-MITF cDNA into mi/mi CMCs. Five micrograms of total RNA from +/+ CMCs, mi/mi CMCs, and mi/miCMCs overexpressing +-MITF were fractionated on 1% agarose gels and subjected to hybridization with the MITF, Gr B, and TPH probes. The +-MITF cDNA introduced into mi/mi CMCs appeared to be transcribed abundantly. Reprobing with the β-actin and MC-CPA probes showed that equal loading had been achieved.

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