Induction of expression of mRNA for RANK in hemopoietic cells by FL.

(A) Expression of mRNA for RANK in bone marrow cells incubated with FL. Expression of RANK mRNA was analyzed by RT-PCR. Bone marrow cells nonadherent after 6 hours without cytokine were incubated for 3 days with and without FL (100 ng/mL) or M-CSF (50 ng/mL) before extraction of RNA from adherent cells. (B) Expression of mRNA for RANK in op/op spleen cells incubated with FL. Spleen cells were incubated with and without IL-3 (1 ng/mL), M-CSF (50 ng/mL), or FL (100 ng/mL) for 3 days. RNA was extracted from adherent cells and analyzed by RT-PCR. Results are from 2 separate experiments.