Flow cytometry analyses of human transduced hematopoietic cells after in vitro culture.

After 6 weeks, coculture with the MS-5 murine stromal cell line, initiated with sorted EGFP⁺ placental CD34⁺cells, transduced with LZRS-EGFP (top) or LZRS-Ik6-EGFP (bottom), were stained with antihuman mAbs against CD10, CD19, and CD33 conjugated with PE. In test cultures initiated with sorted CD34⁺ cells transduced with the LZRS-Ik6-EGFP retroviral vector, a small percentage of cells expressing CD10 and CD19 can be identified; residual B cells in these cultures expressed low levels of EGFP.