Fig. 10.
Sphingosine 1-phosphate treatment does not affect Bcl-2, Bcl-xL, or Mcl-1 levels in Jurkat, U937, and HL-60 cells.
Extracts from Jurkat (A), U937 (B), or HL-60 cells (C) treated for the indicated times with 5 μM S-1P were subjected to 15% SDS-PAGE and immunoblotted with anti-Bcl-2, anti-Bcl-xL, anti-Mcl-1, and actin, as described in Figure 1. Similar results were obtained in 3 independent experiments.