Retroviral transduction of MPCs.

MPCs were subcultured at a 1:4 dilution and then subjected to 2 sequential transductions 24 hours apart with a gibbon ape leukemia virus (GALV)–pseudotyped MFG-eGFP vector. Transduction efficiency was examined by FACS analysis for eGFP. Shown is the result of a representative example of more than 10 experiments on day 3 after transduction and again after 22 additional population doublings.