Differentiation of MPCs from cultures supplemented with 10% FCS or without FCS.
CD45−GlyA− cells were expanded on FN in expansion medium with EGF and PDGF-BB and with 2% FCS, with 10% FCS, or with no FCS but with 10 ng/mL IGF for 25 cell doublings. Cells were replated at MPCs plated at 2 × 104/cm2 under osteoblast, skeletal muscle, and endothelial conditions for 14 days. Cells were analyzed by FACS for expression of bone sialoprotein, fast-twitch myosin, and vWF (thick line) compared with IgG control phenotype (thin line). Differentiation to the osteoblast but not myoblast or endothelial lineage was observed for cells cultured with 10% FCS. Differentiation to osteoblasts, skeletal myoblasts, and endothelium is seen for cells cultured without FCS but with IGF. A representative example of 3 experiments is shown.
