Northern blot analysis of TfR expression in hematopoietic cell lines.

Total RNA (15 μg) from Raji (B lymphoid), MOLT-16 (T lymphoid), U937, NB4, HL-60, KG-1, KCL22 (myeloid leukemia), HEL-R, OCI-M1, KU-812-F, and K562 (erythroid leukemia) was used to analyze TfR1, TfR2, and GAPDH expression. Levels of TfR1expression in Raji and U937 were relatively low, but their expression was confirmed by a longer autoradiography as well as by a prior study.4