Effect of Gly208Ser on the interaction of GATA-1 and FOG in vivo.

NIH3T3 cells were cotransfected with a luciferase reporter gene construct, the wild-type or Gly208Ser form of GATA-1, as indicated (250 ng in pcDNA3) and increasing amounts of a FOG expression vector (0, 50, 100, and 200 ng pMT2FOG, with a higher level for each pair of lanes from left to right). Results are expressed as fold repression of luciferase activity relative to that seen in the absence of FOG (baseline adjusted to 1.0).