Role of CD59 expression in R-CDC.

(A) Expression of CD20 and CD59 in cells from nonsensitive (■) and sensitive (▪) patients. (B) Effect of CD55 and CD59 blockage on R-CDC. Cells from 9 B-CLL patients were incubated for 24 hours with 50 μg/mL rituximab and 10% AB (R+AB) alone or combined with anti-CD55 and/or anti-CD59. Cell viability was determined by annexin V binding as described in “Patients, materials, and methods.”