ROS production induced by rituximab+AB and effect of ROS scavengers.

(A) Cells from a representative CD20⁺⁺ patient with MCL were incubated for 24 hours in the presence (dashed line) or absence (solid line) of 50 μg/mL rituximab and 10% AB serum (R+AB) and stained with DHE. (B) Cells from a representative CD20⁺⁺patient with B-CLL were incubated for 20 hours with ROS scavengers (NAC, 25 mM; Tiron, 5 mM) in the absence or presence of rituximab and AB (R+AB). ROS scavengers were added one hour prior to the addition of rituximab and AB serum. Cell viability was determined by staining with annexin V binding. * Indicates P < .05. (C) The effect of ROS scavengers on ΔΨm and ROS generation was quantified after 20 hours by dual staining with DIOC₆[3] and DHE.