Overexpression of cyclin D3 in a case of CD5+ DLBCL.
(A) Northern blot; 30 μg total RNA was used per lane. Upper panel probe CCND3 mRNA, lower panel GAPDH as loading control. RNA from patient 1 (lane 1) showed overexpression ofCCND3 in comparison to DLBCL cell line MD903 with t(3;14)(q27;q32) (lane 2), DLBCL cell line CTB-1 with t(3;14)(q27;q32) (lane 3), mantle-cell NHL cell line Granta 519 with t(11;14)(q13;q32) (lane 4), transformed SLVL cell line Karpas 1718 (lane 5), and B-ALL (FAB L3) cell line KHM-10B with t(8;22)(q24;q11) (lane 6). Arrowheads indicate 2.0-kb CCND3 transcript. (B) Western blot analysis of CCND3 protein. Total cell extracts (30 μg) from patient 1 were subjected to Western blot and probed with Mabs to cyclin D3, p27Kip1, and tubulin (lower panel) as a loading control. Lane 1, patient material; lane 2, HUT78 T-NHL cell line; lane 3, CEMO cell line; and lane 4, RPMI8226. Arrow indicates 32-kd cyclin D3 and 27-kd p27Kip1 proteins.
