Gut IELs in CCR9-deficient mice.

(A) Analysis of tissue sections allowed us to determine the number of total IELs and to normalize the number to 100 epithelial cells.29 (B) Comparison of the various CD3⁺IEL subsets found in CCR9-deficient and in wild-type mice. IELs were labeled with the specified combination of antibodies and analyzed by flow cytometry. (C) IELs from CCR9-deficient or wild-type mice were analyzed by 2-color flow cytometry for the expression of TCRδ versus CD3. The percentage of TCRγδ⁺ cells is indicated.