SIN-lentiviral constructs.

Vector series driven by the α-spectrin promoter, S (A), the ankyrin-1 promoter, K (B), the ζ-globin promoter Z (C), the β-globin promoter, B (D), and the CMV promoter/enhancer (CMV) (E). RRE designates Rev-responsive element; SD, splice donor; SA, splice acceptor; and H, T, I8 represent the HS-40, GATA-1 ARE, and intron 8 enhancer from eALAS gene, respectively. W represents WPRE and G represents GFP. (F) Southern blot analysis of transduced 293A cell pools (restricted with EcoRI and XhoI, enzymes that flank the transcriptional unit and probed with GFP) shows appropriate single proviral bands in all (including H2BGW, not shown) except for HSGW that shows an additional smaller rearranged band of 1.5 kb. The expected size of the proviral bands ranged between 1.8 and 2.5 kb for all other erythroid vectors and 1.1 kb for the CMV-G vector.