Expression of vectors in primary human cells.

The ratio between MFI of transduced and nontransduced cells was used to determine the fold-increase in MFI (y-axes). Fold increase in MFI of GFP in the progeny of primary human CD34⁺ cells (mean ± SD; n = 4) in (A) pooled transduced BFU-Es and (B) MFI from individual BFU-Es (40 BFU-E/vector). (C) FACS analyses of GFP expression (shown on the x-axes) in erythroid (GlyA⁺) and myeloid (CD13⁺) liquid culture differentiation (as described in “Materials and methods”) transduced with the key vectors. Myeloid and erythroid lineage staining is shown on the y-axes and the proportion of GFP⁺ cells annotated in the respective quadrants.