IL-7 and IL-15 increase c-Myb protein levels in interleukin-starved SeAx cells.

(A) Effects of 10 IL-7. M, marker. Lane 1, 48-hour interleukin-starved SeAx cells. Lane 2, 54-hour interleukin-starved SeAx cells. Lane 3, 54-hour interleukin-starved SeAx cells after 6-hour addition of IL-7. Lane 4, 60-hour interleukin-starved SeAx cells. Lane 5, 60-hour interleukin-starved SeAx cells after 6-hour addition of IL-7. Lane 6, 72-hour interleukin-starved SeAx cells. Lane 7, 72-hour interleukin-starved SeAx cells after 6-hour addition of IL-7. Lane 8, 96-hour interleukin-starved SeAx cells. Lane 9, 96-hour interleukin-starved SeAx cells after 6-hour addition of IL-7. CCS, splice form initially designated as the complete coding sequence; X, unknown c-Myb splice form. (B) Effects of 10 IL-15. M, marker. Lane 1, 48-hour interleukin-starved SeAx cells. Lane 2, 54-hour interleukin-starved SeAx cells. Lane 3, 54-hour interleukin-starved SeAx cells after 6-hour addition of IL-15. Lane 4, 60-hour interleukin-starved SeAx cells. Lane 5, 60-hour interleukin-starved SeAx cells after 6-hour addition of IL-15. Lane 6, 72-hour interleukin-starved SeAx cells. Lane 7, 72-hour interleukin-starved SeAx cells after 6-hour addition of IL-15. Lane 8, 96-hour interleukin-starved SeAx cells. Lane 9, 96-hour interleukin-starved SeAx cells after 6-hour addition of IL-15. (C, D) Corresponding experiments with c-myc gene not regulated by IL-7 and IL-15 (negative control).