Binding of STAT factors to a bcl-2 gene promoter element in CTCL cells.

(A) IL-7 and IL-15 stimulate the binding of STAT factors to abcl-2 gene promoter element. Lane 1, unstimulated PBLs. Lane 2, unstimulated SeAx cells. Lane 3, IL-7–stimulated SeAx cells. Lane 4, IL-15–stimulated SeAx cells. (B-D) Identification of the STAT factors binding to the bcl-2 gene promoter element. (B) IL-7– and IL-15–treated SeAx cells (10 U each). (C) Untreated MyLa 2059. (D) Untreated HUT78 cells. Lane 1, nuclear extract from PBLs. Lanes 2 to 8, nuclear extracts from CTCL cell lines. Lane 2, no antibody added. Lane 3, STAT1 antibody. Lane 4, STAT2 antibody. Lane 5, STAT3 antibody. Lane 6, STAT4 antibody. Lane 7, STAT5 antibody. Lane 8, STAT6 antibody.