![Fig. 4. Morphologic abnormalities in AMs of young Op/Op mice normalize with age. / (A-D) AMs from Op/Op mice or littermate controls were obtained by BAL, formed into sediments by cytocentrifugation, stained with Diff-Quick, and evaluated by light microscopy. (E-F) AM cell diameter was measured using Metamorph software. BAL cells consisted of more than 95% AMs in all mice, and they uniformly and strongly stained for Mac-3 antigen in samples from Op/Op mice (aged 20 ± 0 or 122 ± 14 days [A,C]) and littermate controls (aged 20 ± 0 or 120 ± 16 days [B,D]). In young mice, AM cell diameter was smaller in Op/Op mice than in littermate controls (105 106 cells counted in each group; P < .0001). In older mice,AM cell diameter in Op/Op and control mice was similar (107 118 cells counted in each group; P > .22) (× 100; insets, × 700).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/9/10.1182_blood.v98.9.2845/4/h82111718004.jpeg?Expires=1735508114&Signature=bjVqqq6W72HmTMZlXaSl~FLb9tTe1XwjKUuNOHjwbQwUXbDzhD0urGxgtPaAtn-tZZZSb7rMSZy6xYqbXM~n~FWSNLAbuMrT1SWT0WZA6Iza5DAiCN3rfwFRxxHce-KeIwI9n5pPCz6AkvZG6ltEjSQP2b-piFDyb1XuUvHfm0sKlVwLjNvQBf4fwaJOJ6GtrKDG6w2ZKDunfMmzAKrcO8MtIrda7BGmSQlCnxUZfNXK5RnIOOTBu60Y9QXf6cGuOV5v279u6S0ljbRjhiLZ4WlaJ646sF3LuBe7WDQtcpRbuo-mhQfNE9gTTbq~44b6zuaX4UYlOvSw0d-aQqKcIg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Morphologic abnormalities in AMs of young Op/Op mice normalize with age.
(A-D) AMs from Op/Op mice or littermate controls were obtained by BAL, formed into sediments by cytocentrifugation, stained with Diff-Quick, and evaluated by light microscopy. (E-F) AM cell diameter was measured using Metamorph software. BAL cells consisted of more than 95% AMs in all mice, and they uniformly and strongly stained for Mac-3 antigen in samples from Op/Op mice (aged 20 ± 0 or 122 ± 14 days [A,C]) and littermate controls (aged 20 ± 0 or 120 ± 16 days [B,D]). In young mice, AM cell diameter was smaller in Op/Op mice than in littermate controls (105 106 cells counted in each group; P < .0001). In older mice,AM cell diameter in Op/Op and control mice was similar (107 118 cells counted in each group; P > .22) (× 100; insets, × 700).
