Immunofluorescence for SP- and NK-A–binding sites in BM stroma.

Cells were stimulated with 10 ng/mL SCF and/or 10 nM SP(1-4) for 24 hours. After this, cells were labeled with biotin-SP or biotin–NK-A and then developed with FITC-avidin. Unstimulated (A,C); SCF-stimulated (B,D) consecutive stimulation with SCF and SP(1-4) (E-F).