Western blot analysis of RANK, OPG, and RANKL expression in cultured H/RS cells and primary lymphoid tumors.

(A) H/RS cell lines show that all cell lines expressed RANKL and OPG. RANK was predominantly expressed in HDLM-2 and L-428. The remaining 2 cell lines also expressed RANKL, but at very low levels. All cell lines expressed RANK and RANKL at the mRNA level (data not shown). (B) Primary lymphoid tumors of different phenotypes (B, T, or null) show that RANK was not expressed in these tumors, whereas all tumors expressed RANKL and OPG.