RANKL activates NF-κB in H/RS cell lines.

Incubation of these H/RS cell lines with RANKL (1 μg/mL) activated NF-κB, as shown by the EMSA method. NF-κB activation was most prominent in the HDLM-2 cell line. Activation was detected as early as 10 minutes after incubation with RANKL and lasted for up to 3 hours.