Effect of RANKL on the survival of H/RS cell lines.

(A) H/RS cells were incubated with increasing concentrations of RANKL for 24 hours (left panel), or they were treated with 1 μg/mL RANKL for 24, 48, or 72 hours (right panel). RANKL had no significant effect on cell survival or proliferation. (B) Effect of endogenous RANKL on the survival of H/RS cell lines. H/RS cell lines were incubated with increasing concentrations of soluble RANK, OPG, or CD30 receptors for 24 or 48 hours. The 2 cell lines that expressed high levels of RANK were examined, HDLM-2 (left panel) and L-428 (right panel). Blocking the interaction between endogenous RANKL and its receptor did not have a significant effect on the survival of these cell lines. Data shown represent average results of 3 independent experiments. No differences were observed when cells were incubated with soluble receptors for 24 or 48 hours. Soluble CD30 was used as the control because these cell lines do not express CD30L.