RANKL induces the expression of several cytokines in H/RS cell lines.

Cell lines were incubated with RANKL (1 μg/mL) for 24 hours. Total RNA was extracted and subjected to RNase protection assay. (A) RANKL up-regulated IL-8 mRNA expression in the HD-MYZ cell line. (B) RANKL up-regulated the mRNA expression of IL-15, IL-9, IL-13, and IL-13 receptors in several cell lines. Table 1 shows the quantitative relative expression of cytokine mRNA by using the GAPDH signal as a control for the amount of input RNA.