American Society of Hematology

Fig. 1.

$Fig. 1. Proliferation of 1,000 sorted cells in the serum-free pre-CFU assay. (A) CD34+ cells from five normal donors and five CML patients were cultured in SCF alone or in 4HGF. Cell counts for normal CD34+ cultured in SCF alone remain below the limit of detection and so are represented by a dotted line. (B) CML CD34+ from three patients were subfractionated into CD38+/−and cultured in SCF alone or in 4HGF. CD34+CD38+/− cells from three normal donors showed no discernible proliferation in this assay and are not plotted. (A) □, CML in SCF; ○, CML in 4HGF; •, normal in SCF; ▪, normal in 4HGF. (B) ▪, CD34+CD38+ in SCF; •, CD34+CD38− in SCF; □, CD34+ in 4HGF; ○, CD34+CD38− in 4HGF.$

Proliferation of 1,000 sorted cells in the serum-free pre-CFU assay. (A) CD34⁺ cells from five normal donors and five CML patients were cultured in SCF alone or in 4HGF. Cell counts for normal CD34⁺ cultured in SCF alone remain below the limit of detection and so are represented by a dotted line. (B) CML CD34⁺ from three patients were subfractionated into CD38^+/−and cultured in SCF alone or in 4HGF. CD34⁺CD38^+/− cells from three normal donors showed no discernible proliferation in this assay and are not plotted. (A) □, CML in SCF; ○, CML in 4HGF; •, normal in SCF; ▪, normal in 4HGF. (B) ▪, CD34⁺CD38⁺ in SCF; •, CD34⁺CD38⁻ in SCF; □, CD34⁺ in 4HGF; ○, CD34⁺CD38⁻ in 4HGF.

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