(A) Dose-dependent zymosan-induced dermal neutrophil accumulation. Colorimetric determination of tissue myeloperoxidase content (OD 450 nm) of mouse ears was determined 8 hours after subcutaneous injection of the indicated amount of zymosan. n = 2 animals per group. (B) Time-dependent zymosan-induced dermal neutrophil accumulation. Tissue myeloperoxidase content of mouse ears, measured as described above and quantitated as units of myeloperoxidase per ear, was determined at the indicated time after the intradermal injection of 400 μg zymosan. n = 4 animals per group.