Immunofluorescence photomicrographs of vascular selectin expression in zymosan-injected ears in wild-type mice. Tissue was prepared for immunofluorescent microscopy 6 hours after subcutaneous zymosan injection and intravenous administration of R-phycoerythrin–conjugated anti–E-selectin antibody and fluorescein-conjugated anti–P-selectin antibody. Photomicrographs (original magnification × 400) were taken adjacent to a subcutaneous zymosan deposit and represent the fluorescence associated with a single tubular, branching vascular structure as viewed with appropriate filters for (A) R-phycoerythrin (anti–E-selectin) or (B) fluorescein (anti–P-selectin).