α2 allele genotyping using BglII/Nde I digestion. The region of the α2 gene encompassing the Bgl II and Nde I sites was amplified from genomic DNA as described in Materials and Methods. The 600-bp PCR product was digested with Bgl II (A) or Nde I (B), and the resulting products were analyzed by agarose gel electrophoresis. Lanes 1 and 2, homozygous (allele 1); lane 3, heterozygous (allele 1, allele 3); lane 4, heterozygous (allele 1, allele 2); lanes 5 and 6, homozygous (allele 2); lane 7, control sequence 807C/837C/873G; lane 8, control sequence 807T/837T/873A; lane 9, molecular weight (MW) λHind III/◊X174Hae III; lane 10 (B only), heterozygous (allele 2, allele 3). The 807, 837, and 873 designations refer to the genotype as determined by Southern dot blot analysis of genomic DNA.9 Size of MW markers (in bp) is indicated to the right of the figure.
