(A) Expression of the P falciparum G6PD gene in parasites grown in normal and deficient E. Total P falciparumRNA (strain HB3B) was analyzed by Northern blot using K2O2 as a probe (see Materials and Methods). Approximately 4 μg of total RNA extracted from parasites grown in normal (N) and deficient (D) E at ring (RPE) and trophozoite (TPE) stage of the first growth cycle. Illustrative lanes are labeled at the top with the respective G6PD status. (B) Expression of the P falciparum G6PD gene in peripheral blood of patients infected with P falciparummalaria. Total RNA was extracted from the peripheral blood of two P falciparum–infected G6PD normal patients and analyzed as indicated above. Patient 1: Total RNA extracted from 2 mL of peripheral blood from a patient with a parasitemia of 0.25% RPE, corresponding to approximately 1.8 × 107 parasitized E. Patient 2: Total RNA extracted from 3 mL of peripheral blood from a patient with a parasitemia of 0.6% RPE, corresponding to approximately 8 × 107 parasitized E. RPE: 4.5 μg of total RNA extracted from in vitro culture (ring stage) of strain C1O.
