Fig. 4.
Effect of ASA or ionomycin pretreatment of T cells on [Ca2+]i mobilization induced by the anti–β2-integrin MoAb KIM127. Cells were incubated with 300 μg ASA, 50 nmol/L ionomycin, or medium for 30 minutes at 37°C, washed, and then tested for mobilization of [Ca2+]i. Arrow indicates addition of KIM127 MoAb (medium [•], ASA [◊], and ionomycin pretreatment [□]) or its solvent (control [⧫]). Data are expressed as the mean SEM of five determinations.