Detailed flow cytometric analysis of the various hematopoietic lineages in bone marrow and spleen of engrafted NOD/SCID. Plots are from a representative experiment in which animals were transplanted with 15 × 10⁶ CD34⁺ cells and were analyzed after 6 to 8 weeks. (A) Distribution of B-cell markers CD10 and CD20 on all nucleated cells. (B) Distribution of heavy and light-chain cell-surface immunoglobulin on CD19⁺ human B cells (mean ± SEM of n = 4 to n = 7). All differences between BM and SPL were highly significant (P < .001). (C) Distribution of HLA-DR and CD33 (circles indicate activated HLA-DR⁺and resting HLA-DR⁻ granulocytes) among CD45⁺ cells, (D) expression of CD11b and CD16 on CD45⁺ cells, and (E) expression of human Glycophorin A (Gly A) on CD45⁻ nucleated and enucleated cells. 40,000 events per analysis were recorded.