Analysis of systemic (A and B) and target organ (C and D) GVHD after BMT. CBA mice received syngeneic TCD BM and T cells □, TCD allogeneic (B10.BR) BM alone ▩, or with the addition of 1 × 106 T cells ▪, as described in Materials and Methods. Recipients of allogeneic BM and T cells developed significant GVHD as determined by weight loss (A) and clinical score (B), compared with recipients of syngeneic BMT (**P < .01). The extent of acute GVHD was also assessed 6 weeks after BMT by histopathologic evaluation of target organ tissue as described in Materials and Methods. Six weeks after transplant, recipients of allogeneic BM and T cells developed significant tissue injury in the liver (C) and oral mucosa (D) compared with syngeneic BMT controls (**P < .01). Transplantation of allogeneic TCD BM alone completely prevented the development of GVHD; weights, clinical scores, and target organ pathology scores of these animals were indistinguishable from syngeneic controls. Data are expressed as mean ± SEM. (A and B, n = 12 to 15 per group; C and D, n = 6 to 9 per group)
