Fig. 4.
Comparison of the differentiation-inducing activities of 1,25D3 and R0-25-9716. (A) NBT reduction activities. HL-60 cells were cultured with various concentrations (10−11 to 10−7 mol/L) of either 1,25D3 or Ro 25-9716 for 3 days and differentiation was determined by NBT reduction. (▧), 1,25D3; (▪), Ro 25-9716. (B) Expression of CD11b antigens on HL-60 cells. Cells were treated for 3 days with different concentrations (10−9 to 10−7 mol/L) of either 1,25D3 or Ro 25-9716, and then analyzed by fluorescence-activated cell sorting (FACS) for expression of CD11b. (□), 1,25D3; (⌾), Ro 25-9716.
