Fig. 2.
IFN inducibility of transfected chimeric Ly-6A/E deletion constructs (shown in Fig 1). Stable clones derived from A20-2J cells were analyzed by cell surface staining with MoAb 34-11-3 directed specifically against Ly-6A antigen. Cells were mock treated or treated with IFN-α/β (1,000 U/mL for 24 hours) or IFN-γ (500 U/mL for 24 hours). Plain lines indicate cells stained with secondary antibody only as control (FITC-conjugated rabbit anti-mouse Ig); bold lines indicate cells stained with 34-11-3 followed by secondary antibody.