FAA and FAC do not interact. (A) Immunoprecipitation experiments using extracts derived from metabolically labeled 293 cells that were transiently transfected with the indicated vectors expressing FAA or FAC. Extracts were prepared in RIPA buffer. (B) Immunoprecipitation of FAA and FAC using extracts derived from 293 cells transiently expressing FAA, FAC, or FAA and FAC as indicated. Extracts were prepared in medium salt buffer. Arrowheads indicate the heavy and light chains of the antibodies. (C) Coprecipitation experiments with in vitro–translated³⁵S-methionine–labeled FAA and FAC under low salt conditions. Total reticulocyte lysate was loaded in lanes marked FAA-lysate and FAC-lysate; control indicates total lysate without template DNA. Full-length FAA and FAC are indicated.