American Society of Hematology

Fig. 2.

$Fig. 2. Expression of Bcl-2 family proteins in freshly isolated neutrophils. Expression of Bcl-2 (A), Bcl-XL (B), Bax (C), and Mcl-1 (D) were determined by Western blotting as described in Materials and Methods with PBMNC shown as a positive control for Bcl-2, Bcl-XL, and Mcl-1. Blots were stained with Ponceau S and the actin-stained band is shown for each blot to indicate equal protein loading per lane. Histones from cell fractionation are shown in (C) with identical samples used in (D). Each blot is representative of at least two separate experiments.$

Expression of Bcl-2 family proteins in freshly isolated neutrophils. Expression of Bcl-2 (A), Bcl-X_L (B), Bax (C), and Mcl-1 (D) were determined by Western blotting as described in Materials and Methods with PBMNC shown as a positive control for Bcl-2, Bcl-X_L, and Mcl-1. Blots were stained with Ponceau S and the actin-stained band is shown for each blot to indicate equal protein loading per lane. Histones from cell fractionation are shown in (C) with identical samples used in (D). Each blot is representative of at least two separate experiments.

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