Expression of Mcl-1 in neutrophils treated to delay apoptosis. Expression of Mcl-1 in freshly isolated neutrophils and neutrophils cultured for 6 hours (A) and 12 hours (B) in the conditions indicated, were determined by Western blotting as described in Materials and Methods. Ponceau S–stained actin is shown for comparison of protein loading per lane. Each treatment shown is representative of at least two separate experiments.