Aci I RFLP for the identification of the 271C → T mutation. DNA was amplified using the FY3 and FY4 primers. Restriction fragments were separated on 1% agarose gel. (A) Schematic diagram of fragments generated by Aci I digestion of the DARC fragment encompassing nt 271. (B) RFLP patterns of DNA from samples with the indicated phenotypes, identified by antisera, and genotypes, determined by Ban I and Sty I (FY*B = wild-type GATA; FY*B− = GATA mutation). Lanes: 1 through 3, Fy(a−b−)FY*B−/FY*B−; 4, Fy(a−b−)FY*B/FY*B−; 5 through 7, Fy(a+b−)FY*A/FY*B−; 8, Fy (a+b−)FY*A/FY*B.