Expression of TCR Vα12 in isolated Vα2⁺ PBLs. (A) Vα2 expression in PBMCs. (B) Vα2 expression after immunomagnetic isolation. (C) Gating of CD25^– and CD25^hi cells within the Vα2⁺ population. (D) Vα12 expression in Vα2⁺CD25^– and (E) Vα2⁺CD25^hi cells. In panels A, B, D, and E, the cutoff point between positive and negative cells is indicated. A representative example of 7 donors is shown.