TLR stimulation induces the expression of SOCS. Freshly isolated human CD14⁺ monocytes were stimulated with 10 ng/mL or 30 ng/mL LPS and analyzed for expression of (A) SOCS-1, (B) SOCS-3, and (C) CIS by RT-PCR at the indicated time points (mean of duplicate determinations from 1 of 3 typical donors). (D) Murine bone marrow cells (Balb/c) were stimulated with 100 ng/mL LPS immediately after preparation and were analyzed for expression of SOCS-1, SOCS-3, and CIS by RT-PCR at the indicated time points (n = 4, mean+SD). Expression of SOCS1 (E) or SOCS-3 (F) was determined in monocytes stimulated with 10 μg/mL Pam₃CysSK₄,1 μg/mL FSL, 50 μg/mL poly[(I:C)], or 10 ng/mL LPS for 7 hours (1 representative of 3 experiments).