A pDC population in the BM of Ik^L/Lmice. (A) CD11c⁺ BM cells were gated and analyzed for B220 and 120G8 expression. Numbers indicate percentage of cells in the gated contour plots. (B) 120G8⁺ spleen and BM cells were analyzed for their expression of the indicated surface antigens. The pDC population is indicated by the white histograms. Gray histograms indicate the isotype controls. Results are representative of 3 experiments. (C) Sorted CD11c⁺ 120G8⁺ BM cells were cultured with poly I:C, inactivated influenza virus, or CpG D19 for 24 hours, and supernatants were tested for the indicated cytokines. Results are representative of 2 experiments, on pools of cells from 5 mice/genotype per experiment. Means and SDs are shown for 1 representative experiment.