Specific immune responses against peptides derived from PRAME in patients with AML. A high frequency of specific T-cell responses was found against the PRAME-derived peptide P3. This patient with AML showed a strong immune response for the PRAME-P3 peptide in ELISPOT analysis for IFN-γ (A) and granzyme B (B). No immune responses were observed for the other PRAME-derived peptides tested (P1, P2, P4) when compared with the cross-priming experiment against an irrelevant peptide (MAGE3). A strong immune response was detected for the IMP peptide used as a positive control (x-axis: counted spots per 10 000 CD8⁺ T cells are indicated). Error bars indicate standard deviation.