Dose-dependent cellular immune responses to the G250/CA9-derived peptide G2. A dose-dependent specific immune reaction against G250/CA9-G2–pulsed T2 cells could be detected analyzed by ELISPOT assays for IFN-γ and granzyme B. The optimal dose of 10 μg peptide G250/CA9-G2 per milliliter was used in consecutive assays. For the TAAs PRAME and RHAMM/HMMR,⁸  similar results were found showing also an optimal peptide dose of 10 μg/mL.