Implication of PLD pathway in mTOR activity. (A) PLD activity was measured in FL cell lines and compared to B cells isolated from healthy donors (BL) and from B-CLL patients (B-CLL) as described in “Materials and methods.” Results are the mean ± SD of 3 independent experiments. (B) PLD-dependent activation of mTOR was evaluated using antiphospho-p70S6K (Thr389) antibody in Karpas-422 and RL cells treated or not with 1-butanol or 3-butanol at 0.3% during a time course. p70S6K was used as control of protein expression. CT corresponds to untreated FL cells and results are representative of 3 independent experiments. (C) Syk-dependent PLD activation was evaluated by treating FL cell lines with piceatannol at 50 μM for 2 hours and measuring PLD activity as described in “Materials and methods.” Results are the mean ± SD of 3 independent experiments.