Expression of PTPγ in peripheral blood, hematopoietic tissues, and differentiated monocytes. Number of copies of PTPRG mRNA per 106 copies of ACTB estimated by QPCR analysis in peripheral blood cells of individual donors (monocytes, n = 8; PBL, n = 6; PMN, n = 5; MDCs, n = 4; plasmacytoid DCs, n = 4) (A) and in primary and secondary hematopoietic tissue (spleen, n = 3 subjects; thymus, n = 2 subjects; tonsil, n = 2 subjects; lymph nodes, n = 3 subjects; bone marrow, n = 8 subjects) (B). QPCR analysis of PTPγ expression in monocytes (n = 8 subjects) induced to differentiate to macrophages (moMΦ, n = 5 subjects) or dendritic cells (moDC, n = 9 subjects) in the presence or absence of LPS (n = 7 subjects) and CD40L (n = 9 subjects) (C). Statistically meaningful P values are indicated for panels A and C (calculated with unpaired t test with Welch correction). Graphs A and C are box plots in which median, 25th percentiles, 75th percentiles, minimum values, and maximum values are presented as vertical boxes and lines. In panel B, error bars represent standard deviation (SD).
